To make the test even more challenging, Ampliqon Taq polymerase was kept in a buffer without glycerol. Glycerol is a cryoprotectant, and are normally part of the storage buffer where it serves to protect Taq polymerase during freezing conditions. Analysis was done using real-time PCR. Samples were diluted to a concentration, where the amount of Taq polymerase was limiting, thereby allowing to monitor changes in enzyme activity.
Starting with the same dilution, a standard curve for Ampliqon Taq polymerase activity was prepared from the sample without freeze-thaw cycles. Ampliqon Taq DNA polymerase is a remarkable thermostable enzyme. At lower temperatures, Taq polymerase is stable for even longer time. However, if you forget your enzyme on your lab bench even for several days, no harm is done due to the high stability of Taq polymerase at room temperature.
Ampliqon Taq DNA polymerase offers a perfect combination of heat resistance, robustness, specificity, sensitivity and yield and is commonly agreed to be one of the best polymerases available. Request more information. I would like to request more information about:.
Toggle navigation. Newsletter signup Contact us Dansk. Hot Start. Lyophilized PCR. Glycerol Free Products. Because significant amounts of a sample of DNA are necessary for molecular and genetic analyses, studies of isolated pieces of DNA are nearly impossible without PCR amplification.
Often heralded as one of the most important scientific advances in molecular biology, PCR revolutionized the study of DNA to such an extent that its creator, Kary B.
Mullis, was awarded the Nobel Prize for Chemistry in PCR is also valuable in a number of laboratory and clinical techniques, including DNA fingerprinting, detection of bacteria or viruses particularly AIDS , and diagnosis of genetic disorders. Next, an enzyme called "Taq polymerase" synthesizes - builds - two new strands of DNA, using the original strands as templates. Magnesium is an essential cofactor for Taq polymerase 3. The concentration of magnesium also matters. As more magnesium is added to a PCR reaction, Taq becomes more active, but also less specific in polymerising only your target DNA strand.
Because of the concentration-dependent role of magnesium, you can find Taq master mixes with magnesium already included as well as mixes that leave it out. The advantage is that you can control the exact concentration of magnesium, so you can experiment to find the most optimal blend of Taq productivity and specificity. Taq has an error rate of around one error per , base pairs 4.
By comparison, Pfu proofreading polymerase is about 10 times more accurate. Standard Taq polymerase can also have problems with amplifying target DNA sequences longer than about 1 kB. Relative to other types of polymerases, Taq has low processivity, which means that its efficiency declines significantly with the length of your amplicon. As a result, Taq is known to produce primer dimers and other unwanted products that can get in the way of downstream applications like cloning. Thankfully, there are specialised forms of Taq polymerase that solve some of these problems.
As a result, you can more easily achieve amplicon yields from high-GC templates, templates longer than 1 kB in length, and samples with moderate concentrations of inhibitors.
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